Accessibility of Myofilament Cysteines and Effects on ATPase Depend on the Activation State during Exposure to Oxidants
Figure 8
The TMR fluorescence of several myofilament proteins is sensitive to the contractile state.
A. TMR scan of ventricular myofibrils briefly treated with TMR under rigor, relaxing or denaturing conditions. B. Dot plot of MHC TMR fluorescence (arbitrary units = AU) from relaxing and rigor conditions. * indicates p<0.05 C. TMR and Sypro scans from myofibrils labeled in rigor solution+Ca2+, or rigor solution for five minutes (100 µmol/L TMR). D. TMR scan of TnC labeled in rigor+calcium for 60 minutes, rigor+calcium for 5 minutes, and rigor without added calcium for 5 minutes (100µmol/L TMR). E. Graph and TMR fluorescence of the fraction of TnC fluorescence in the lower of the two TnC bands, over a range of [Ca2+] (rigor +calcium, 100 µmol/L TMR, 5 minutes).
