High Sensitivity of Giardia duodenalis to Tetrahydrolipstatin (Orlistat) In Vitro
Figure 2
Orlistat inhibits growth of G. duodenalis trophozoites.
Anti-G. duodenalis activity of MTZ (filled circle) and orlistat (blank squares) was determined in a resazurin assay and results are presented as relative percentage of growth of the untreated trophozoites as a control. In addition, the half maximal inhibitory concentrations (IC5024h) are shown. (A) Growth inhibition curve of the laboratory strain WB-C6; the IC5024h of orlistat (IC5024h = 4.3 µM; n = 7) was significantly lower than the IC5024h of MTZ (IC5024h = 11.0 µM; n = 10), p = 0.0034. (B) Growth inhibition curve of the G. duodenalis patient isolate 14-03/F7; the IC5024h of orlistat (IC5024h = of 2.8 µM, n = 4) was significantly lower than the IC50 of MTZ (IC5024h = 6.2 µM; n = 5), p = 0.0079. Data are expressed as mean ± SD. The Mann-Whitney-test was used to evaluate the statistical significance of the differences in IC50 values.
