High Sensitivity of Giardia duodenalis to Tetrahydrolipstatin (Orlistat) In Vitro
Figure 4
Scanning electron microscopy of orlistat-treated Giardia duodenalis trophozoites (WB-C6 strain) showing altered morphology.
(A) Solvent control (DMSO, 0.1% in medium). (B) MTZ, 11 µM (C) Solvent control (ethanol, 0.12% in medium). (D, E, F) Orlistat, 5 µM. Controls (A, C) reveal the typical appearance of trophozoites with their pear-shaped cell body and the characteristic arrangement of the eight flagella. MTZ treated trophozoites (B) possess shorter flagella than the corresponding control trophozoites (A). Orlistat treated trophozoites (D, E, F) appear shrunken in comparison to corresponding control trophozoites (C) and show blebs on their dorsal surface (arrowheads) as well as at the tip of the flagella (arrow) (D). (E, F) In some of the trophozoites orlistat mediated effects (blebs at flagella and dorsal cell surface) are more prominent. Scale bar = 5 µm.
