High Sensitivity of Giardia duodenalis to Tetrahydrolipstatin (Orlistat) In Vitro
Figure 5
Killing of G. duodenalis trophozoites by orlistat.
Giardiacidal effects of orlistat or MTZ were determined for drug concentrations indicated using G. duodenalis strains WB-C6 and 14-03/F7. WB-C6 trophozoites (5×104) and 14-03/F7 trophozoites (1×105) were treated with orlistat and MTZ, respectively, for 24 h and, after drug removal, cultured for another 48 h. Trophozoite numbers were determined every 24 h. (A) Orlistat was used at 5×(21.5 µM), 10×(43 µM), and 15×(64.5 µM) of the IC5024h value for WB-C6. (B) Similarly, MTZ was used at 5×(55 µM), 10×(110 µM), and 15×(165 µM) of the IC5024h concentration for WB-C6. (C) Orlistat was used at 5×(14 µM), 10×(28 µM), and 15×(42 µM) of the IC5024h value for 14-03/F7. (D) Similarly, MTZ was used at 5×(31 µM), 10×(62 µM), and 15×(93 µM) of the IC5024h concentration for 14-03/F7. Data are expressed as mean ± SD of parasite numbers determined in triplicate cultures. One of three experiments with similar results is shown (note the gap on the y-intercept).
