High Sensitivity of Giardia duodenalis to Tetrahydrolipstatin (Orlistat) In Vitro
Figure 6
No interaction effect of MTZ and orlistat in combination on the growth of G. duodenalis trophozoites.
Trophozoites of G. duodenalis, (A) WB-C6 (n = 4) and (B) 14-03/F7 (n = 3), were incubated in the presence of various concentrations of MTZ (0.33–81 µM) or orlistat (0.33–27 µM) alone or in combination. Combination indices (CIs) of MTZ and orlistat were calculated for given drug ratios (MTZ:orlistat) of 1∶1 (filled circle), 3∶1 (open squares), or 1∶3 (open triangle). Results are plotted against the fraction of parasites affected (normalized growth inhibition). Data are presented as mean ± SD of independent experiments (each performed in triplicates). CI values between 0.8–1.2 (shaded area) indicate no interaction of the drugs regarding growth inhibition (i.e. drugs act independently).
