Opposite Effect of Mast Cell Stabilizers Ketotifen and Tranilast on the Vasoconstrictor Response to Electrical Field Stimulation in Rat Mesenteric Artery
Figure 4
Effect of ketotifen and tranilast on neuronal NO synthesis and vasodilation (A) Effect of preincubation with ketotifen or tranilast on basal and EFS-induced NO release in mesenteric segments.
n = 5 animals each group. Results (means + S.E.M.) are expressed as arbitrary units (A.U.)/mg tissue.
*P< 0.05 vs basal; # P< 0.05 vs control. (B) Western blot for nNOS and P-nNOS expression in control, ketotifen- and tranilast-incubated mesenteric segments. Figure is representative of preparations from six samples in each group. Lower panel shows relation between densitometric analyses for P-nNOS vs. nNOS expression. * P< 0.05. (C) Vasodilator response to DEA-NO in control, ketotifen- incubated and tranilast-incubated mesenteric segments. Results (means + S.E.M.) are expressed as a percentage of the inhibition of contraction induced by NA. n = 6 animals each group.
