Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration
Figure 1
Treatment with rPAF-AH decreases CFU numbers in the peritoneal fluid after CLP model.
(A) Mice subjected to CLP were treated with rPAF-AH (1 mg/kg-i.p. 15 minutes after). At 6 h after CLP, the mice were euthanized, and the peritoneal fluid was collected and plated on agar plates. (B) At 24 h after CLP, mice were euthanized, and the peritoneal fluid was collected and plated on agar plates. After infection, mice were euthanized 6 (CLP+Vehicle n = 11 animals; CLP+PAF-AH n = 10) and 24 (CLP+Vehicle n = 6 animals; CLP+PAF-AH n = 6) hours after infection, and the peritoneal fluid was collected. Twelve microliters of the peritoneal fluid from each mouse were serially diluted and plated on agar plates. The data represent three different experiments, and the horizontal line represents the median of CFU count. (*) indicate significant difference (P = 0.05) when compared to vehicle treatment.
