Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration
Figure 2
Treatment with rPAF-AH decreases CFU numbers in the peritoneal fluid after S. Typhimurium or E. coli administration.
(A) Mice injected with S. Typhimurium (5×105) were treated with rPAF-AH (1 mg/kg-i.p. 15 minutes after - S. Typhimurium+Vehicle n = 5 animals; S. Typhimurium+PAF-AH n = 6). (B) Mice injected with E. coli (106) were treated with rPAF-AH (1 mg/kg-i.p. 15 minutes after- E. coli+Vehicle n = 5 animals; E. coli +PAF-AH n = 4). Six hours after infection, the mice were euthanized, and the peritoneal fluid was collected. Twelve microliters of the peritoneal fluid from each mouse were serially diluted and plated on agar plates. The data represent three different experiments, and the horizontal line represents the median of CFU count. (*) indicate significant difference (P = 0.05) when compared to vehicle treatment.
