Processive Pectin Methylesterases: The Role of Electrostatic Potential, Breathing Motions and Bond Cleavage in the Rectification of Brownian Motions
Figure 10
Schematic representation of the potential energy changes during Ec-PME processive catalysis.
Calculated electrostatic binding energy of Ec-PME in complex to the HXM decasaccharide compared with the potential of a hypothetical molecular motor. The solid lines refer to regions of the potential sampled by molecular dynamics while the dashed line describes the final docking of the whole oligomer with the restoration of the alternating monosaccharide residue orientations and the next de-methylesterification reaction (not sampled in molecular mechanics studies). Relative potential units are reported in kJ mol−1 (black) and kbT (red).
