Different Subsets of Macrophages in Patients with New Onset Tuberculous Pleural Effusion
Figure 1
Flow cytometry analyses of macrophages.
Peripheral blood mononuclear cells (PBMCs) and pleural fluid mononuclear cells (PFMCs) were obtained from individual subjects and stained with PerCP-anti-CD14 and Alexaflour-conjugated-anti-CD163 or isotype-matched IgG. The cells were gated initially on living mononuclear cells (top left) and then on CD14+ macrophages (top right). Subsequently, the numbers of CD14+CD163+ M2-like and CD14+CD163− M1-like macrophages were determined by flow cytometry, based on total numbers of monocytes/macrophages. Data shown are representative charts of flow cytometry and expressed as the mean values of individual subjects (n = 17 for the HC, n = 25 for the patients). The horizontal lines indicate the median values for individual groups.
