Different Subsets of Macrophages in Patients with New Onset Tuberculous Pleural Effusion
Figure 3
Analysis of PB and PF IL-12+ M1 and IL-10+ M2 macrophages.
PBMCs and PFMCs were isolated from individual subjects and stimulated with LPS/PMA/ionomycin in vitro. The cells were stained with Alexaflour-conjugated-anti-CD163, and PerCP-anti-CD14 for 30 min, fixed, and permeabilized, followed by intracellular staining with FITC-anti-IL-10 or PE-anti-IL-12. The numbers of PB and PF CD14+CD163−IL-12+ M1 and CD14+CD163+IL-10+ M2 macrophages in individual subjects were determined by flow cytometry, based on total numbers of monocytes/macrophages. Data shown are representative FACS charts or the mean numbers of each type of cells per ml of PB and PF in individual subjects from two separate experiments. The horizontal lines indicate the median values for each group.
