Development of Pre-Clinical Models for Evaluating the Therapeutic Potential of Candidate siRNA Targeting STAT6
Figure 3
In vitro Caco-2 cell monolayer toxicity testing of STAT6 siRNA.
Caco-2 cell monolayers were exposed to a range of siRNA concentrations (50 – 5000pM) for 6 hours and membrane integrity monitored by the transfer of Lucifer Yellow dye between the basolateral and apical compartments. No significant transfer of Lucifer Yellow dye was noted following treatment with either 372u (a) or 372m (b) compared to the vehicle control (saline). Analysis of siRNA recovered from the apical (c, d) and basolateral (e, f) compartments following treatment, showed that both 372u (c, e) and 372m (d, f) were present at ≤ 4pg/µl, LLOQ = 0.01 pg/µl). Values presented are mean ± S.E.M (n = 3), and P-values represent comparison to the vehicle (saline) control.
