Development of Pre-Clinical Models for Evaluating the Therapeutic Potential of Candidate siRNA Targeting STAT6
Figure 4
Bio-distribution and persistence of STAT6 targeting siRNA in rat lungs following intra-tracheal delivery.
Normal rats were administered 372u or 372m (1 mg/kg, intra-tracheal) and siRNA quantification performed in lung tissue and plasma. Whole lung analysis showed the presence of significantly more 372m after 6 hours compared to 372u (b). 372u (c) and 372m (d) were both distributed throughout the lung 5 minutes after intra-tracheal administration. In addition, plasma concentrations of 372m (f) were significantly higher and persisted for longer than 372u (e). 1 = right accessory lobe, 2 = right cranial lobe, 3 = right middle lobe, 4 = upper left lobe, 5 = mid left lobe, 6 = lower left lobe, 7 = upper right caudal lobe, 8 = mid right caudal lobe and 9 = lower right caudal lobe. Values presented are mean ± S.E.M (n = 3), and P-value represents comparison between 372m and 372u.
