MiR-99a Antitumor Activity in Human Breast Cancer Cells through Targeting of mTOR Expression
Figure 3
mTOR is a direct target of miR-99a in MCF-7 breast cancer cell line.
(A) Targetscan prediction of miR-99a targeting of mTOR at the mTOR mRNA 3′-UTR.(B) miR-99a-mediated directly inhibitory effect of mTOR by luciferase reporter assays in which the expression of the reporter containing mTOR 3′UTR was suppressed by miR-99a, but not in the mutated construct. (C) The miR-99a-mediated suppression of mTOR at both mRNA and protein levels in MCF-7 cells and analyzed by qRT-PCR and Western blot, respectively (P<0.05 compared to control cells). (D) mTOR rescue analysis. The miR-99a-mediated suppression of mTOR in MCF-7 cells was rescued by transfection of mTOR cDNA plasmid without the 3′-UTR.
