MiR-99a Antitumor Activity in Human Breast Cancer Cells through Targeting of mTOR Expression
Figure 5
mTOR mediated miR-99a antitumor activity in breast cancer cells.
(A) mTOR depletion-mediated cell viability and apoptosis. MCF-7 cells were transfected with MOCK, NC and mTOR siRNA respectively. An MTT cell viability assay was performed at 0, 12, 24, 48 and 72 h. Cell apoptosis was analyzed at 72 h post-transfection, as described in Materials and methods. (B) Cell survival analysis by overexpression of mTOR in breast cancer cells. MDA-MB-231 cells were transfected with MOCK, NC and mTOR cDNA plasmid without the 3′-UTR respectively. (C) miR-99a-mediated cell survival and mTOR rescue analysis in breast cancer cells. MCF-7 cells were co-transfected by miR-99a mimics and mTOR cDNA plasmid without the 3′-UTR followed by MTT assay and apoptosis analysis, as described in Materials and methods.
