Growth Inhibitory, Bactericidal, and Morphostructural Effects of Dehydrocostus Lactone from Magnolia sieboldii Leaves on Antibiotic-Susceptible and -Resistant Strains of Helicobacter pylori
Figure 5
The proportion of coccoid versus spiral form.
H. pylori ATCC43504 was cultured in Brucella broth without or with dehydrocostus lactone (DCL) at 1 (4 mg/L) and 2 times (8 mg/L) the minimal inhibitory concentration for 24 (▪) and 48 h (□). Effect of concentration (F = 1363.16; df = 2, 15; P<0.0001) and exposure time (F = 188.66; df = 1, 15; P<0.0001) on conversion of H. pylori to the coccoid form was significant when H. pylori controls were compared to DCL-treared H. pylori. The concentration by exposure interaction was also significant (F = 40.41; df = 2, 15; P<0.0001). DCL caused considerable conversion to the coccoid form (58 versus 30% at 8 and 4 mg/L DCL for 24 h; 94 versus 49% at 8 and 4 mg/L DCL for 48 h).
