Growth Inhibitory, Bactericidal, and Morphostructural Effects of Dehydrocostus Lactone from Magnolia sieboldii Leaves on Antibiotic-Susceptible and -Resistant Strains of Helicobacter pylori
Figure 6
H. pylori ATCC 43504 urease was treated with dehydrocostus lactone (DCL) and (–)-epigallocatechin gallate (EGCG). The UreA (monomer) and UreB (dimer) bands were confirmed by Western blot with antibodies toward H. pylori urease. The UreB band and UreA dimer and monomer bands were not disappeared at 10 mM DCL. However, the UreA monomer band completely disappeared at 0.1 mM EGCG, and the UreA dimer and UreB bands were faint at the same concentration of the compound. Lanes: 1, ATCC 43504 urease; 2, 2 mM DCL; 3, 5 mM DCL; 4, 10 mM DCL; 5, 0.1 mM EGCG; 6, 0.2 mM EGCG; 7, 0.5 mM EGCG.
