Changes in Acetyl CoA Levels during the Early Embryonic Development of Xenopus laevis
Figure 3
Levels of CoASH, acetyl CoA and total short-chain CoA esters during the early embryonic development of Xenopus laevis
. (A) Levels of unesterified CoA (CoASH) and acetyl CoA were measured in neutralised PCA extracts of Xenopus embryos collected at different stages of early embryonic development. Embryonic stages were determined as described by Nieuwkoop and Faber [24]. 30 embryos were collected for the determination of CoA compounds at each stage. The number of independent measurements (in brackets) performed for each stage is as follows: S1 (8); S2 (5); S3 (5); S4 (9); S8/9 (6); S9/10 (9); S12.5/13 (4); S20 (6); S21/23 (3); S27/28 (3); S37/38 (4); S40 (3). Each data point represents the mean +/− SEM. * indicates statistical significance (p<0.05) compared to the immediately preceding stage as determined by Student's t test. (B) Levels of total acid-soluble CoA species, representing combined levels of CoASH and total short-chain CoA esters, were determined after KOH hydrolysis as described in the Materials and Methods section. The number of independent measurements performed for each stage is as follows: S1 (7); S2 (4); S3 (3); S4 (9); S8/9 (6); S9/10 (8); S12.5/13 (4); S20 (6); S21/23 (3); S27/28 (3); S37/38 (4); S40 (3). Each data point represents the mean +/− SEM. * indicates statistical significance (p<0.05) compared to the immediately preceding stage as determined by Student's t test.
