Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp. indica)
Figure 2
Map-based cloning of the Ygl7 locus.
A. Preliminary map of the Ygl7 locus between RM1038 and SFP-3-6 based on analysis of 368 F2 (ygl7/NPB) individuals. Numbers below indicate the genetic distance (cM) between the markers. B. Fine mapping of the Ygl7 locus, which was narrowed down to a 64.8 kb genomic DNA region between RM10106 (2 recombinants) and STS3-1 (6 recombinants) based on 651 F2 individuals. C. Ygl7 locus was covered by two BAC contigs AC135595 and AC137507. D. There were 12 expressed genes identified in the fine mapping of Ygl7's area. E. One candidate Ygl7 gene (Os03g59640) was found to contain one base replacement (1883, T→C) resulting in one amino acid mutation (628, Leu→Ser). F. PCR analysis of the F2 yellowy-green leaf plants from Ygl7 crossed with NPB. Marker, RM16108; P1, Ygl7; P2, NPB; M, 100 bp low ladder; others are individual yellowy-green leaf plants of the F2 from Ygl7 crossed with NPB.
