The Effects of Fluctuations in the Nutrient Supply on the Expression of Five Members of the AGL17 Clade of MADS-Box Genes in Rice
Figure 3
Effect of deprivation and re-supply of phosphate (P) and sulfate (S) on the expression of five ANR1-related genes in rice.
Two-week old rice seedlings grown hydroponically in complete nutrient solution were deprived of P or S or were maintained on complete nutrient supply for 3 d. In the light period on the day of the experiment, one set of the P-starved and S-starved plants were re-supplied with 0.32 mM H2PO4− and 2.1 mM SO42− respectively. Roots were harvested 3 h later from controls: continuous nutrient supply (CK); P-deprived (−P); P-resupply (+P); S-deprived (−S); S-resupply (+S). Total RNA was extracted from roots and qPCR reactions were performed in triplicate for each RNA sample. The mRNA of OsActin was used as the reference. The value of related genes were normalized to its CK control respectively. A Student’s t-test was calculated at the probability of either 5% (*, p<0.05) or (**, P<0.01).
