A Novel marRAB Operon Contributes to the Rifampicin Resistance in Mycobacterium smegmatis
Figure 2
Assays for the Ms6508–Ms6510 co-transcription by reverse transcription PCR.
(A) The operon structure of Ms6508–Ms6509–Ms6510. Primers were designed for assays and indicated by black arrows. (B) Reverse transcription PCR assays for Ms6508–Ms6510 co-transcription. mRNA (DNA-free) was used as negative controls. PCR procedure was as follows: reactions were degenerated at 95°C for 30 s, annealed at 60°C for 30 s, extended at 72°C for 30 s and under 35 cycles.
