A Novel marRAB Operon Contributes to the Rifampicin Resistance in Mycobacterium smegmatis
Figure 5
Construction of the MarR knockout strain of M. smegmatis and southern blot assays.
(A) Schematic representation of the recombination strategy for removing marR from the genome of M. smegmatis. (B) A map of the recombinant vector pMindMs6508KO containing upstream and downstream sequences of marR, and the gene that confers resistance against hygromycin. (C) Schematic representation of the DNA fragments of the wildtype strain and the marR knockout strain treated with the restriction enzyme SalI. The probe is indicated with a black bar. (D) Southern blot assays. A 387 bp probe corresponding to the upstream sequences of marR in M. smegmatis was obtained by PCR and was labeled with digoxigenin dUTP (Boehringer Mannheim Inc., Germany).
