The Citrus Flavone Nobiletin Reduces Pro-Inflammatory and Pro-Labour Mediators in Fetal Membranes and Myometrium: Implications for Preterm Birth
Figure 5
Effect of nobiletin on LPS-induced MMP-9 expression and release in term fetal membranes and myometrium.
(A,B) Fetal membranes and (C,D) myometrium were incubated with or without 10 µg/mL of LPS in the absence or presence 200 µM of nobiletin for 20 h (n = 6 patients per group). (A,C) MMP-9 mRNA expression was analysed by qRT-PCR and normalised to GAPDH mRNA expression. The relative fold change was calculated relative to LPS and data presented as mean ± SEM. *P<0.05 vs. LPS (one-way ANOVA). (B,C) The incubation medium was assayed for pro MMP-9 levels by gelatin zymography. The relative fold change was calculated relative to LPS and data presented as mean ± SEM. *P<0.05 vs. LPS (one-way ANOVA). Zymography from one patient per tissue type is also shown.
