The Citrus Flavone Nobiletin Reduces Pro-Inflammatory and Pro-Labour Mediators in Fetal Membranes and Myometrium: Implications for Preterm Birth
Figure 7
Effect of nobiletin on MMP-9 expression in preterm fetal membranes.
Preterm fetal membranes with histological chorioamnionitis and following spontaneous preterm labour were incubated for 20 h with or without 200 µM nobiletin (n = 9 patients). (A) MMP-9 mRNA expression was analysed by qRT-PCR and normalised to GAPDH mRNA expression. The fold change was calculated relative to basal expression, which was set at 1. Data is displayed as mean ± SEM (one-way ANOVA). *P<0.05 vs. basal expression. (B) The incubation medium was assayed for for pro MMP-9 levels by gelatin zymography. Data was normalised to untreated (basal) levels, which was set at 1. Each bar represents mean ± SEM (one-way ANOVA). *P<0.05 vs. basal release. Zymography from two patients is also shown.
