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Phylogenetic Analysis of Algal Symbionts Associated with Four North American Amphibian Egg Masses

Figure 3

Light microscopic images of cultured strains of A. maculatum algae.

The Oophila strains Hb_cul-rk (A−C) and BB_cul-B (D−F) belong to subclades I and III, respectively. Monotypic cultures displayed at least three different cell types, which include 1) free-swimming biflagellates (A, D), which correspond to zoospores or gametes, 2) cells enclosed within a mother cell wall (B, E), likely representing asexually dividing zoospores, and 3) larger non-motile zygotes (C, F). Scale bars: 10 µm (A−F).

Figure 3

doi: https://doi.org/10.1371/journal.pone.0108915.g003