Ultra High Content Image Analysis and Phenotype Profiling of 3D Cultured Micro-Tissues
Figure 2
Stepwise demonstration of the image analysis method. Scale bar represents 100 micrometer.
(a) Image stack obtained from the Hoechst stained nuclei channel. (b) Image stack obtained from the Rhodamine stained F-actin channel. (c) In-focus 2D image projected from the stacks of Hoechst stained nuclei channel. (d) In-focus 2D image projected from the stacks of Rhodamine stained F-actin channel. (e) Binary nuclear mask after segmentation by Watershed Masked Clustering. (f) Binary cellular mask after segmentation. The subpopulation classification result is also shown here. The green contour represents branched and interconnected complex networks. The red contour represents spherical colonies. (g) Quantitative parameters measured for each well of the 384-well plates. See Methods for further description.
