The Kinetochore Protein Kis1/Eic1/Mis19 Ensures the Integrity of Mitotic Spindles through Maintenance of Kinetochore Factors Mis6/CENP-I and CENP-A
Figure 2
Isolated mutants showing defects in the organization of microtubules or of the nuclear envelope.
GFP-Atb2 (green), Nup40-mCherry (red), and Sfi1-CFP (blue) of isolated mutants are shown. All mutants were observed after increasing the temperature to 36°C for 3–6 h. Cells displaying the typical phenotype are marked with white arrowheads. The numbers of mutants are also shown. Mutants were categorized according to their phenotype (A–L), and a brief explanation of each phenotype is as follows. (A) Monopolar spindle. (B) The middle region of the spindle showed a dim GFP signal. (C) Accumulation of cells within the metaphase spindle. (D) The spindle was bent in anaphase. (E) Extremely short microtubules. (F) The number of microtubule bundles was fewer than in wild-type cells. (G) Microtubules were elongated and curved at cell tips. (H) Microtubules formed in the nucleus during interphase. (I) Microtubules were tethered around the cell tip. (J) The nuclear envelope was fragmented. (K) Cells had more than one nucleus. (L) Multi-septated cells. A differential interference contrast image of the outlined cell is also shown. Red arrowheads indicate septa. Scale bar: 5 µm.
