The Kinetochore Protein Kis1/Eic1/Mis19 Ensures the Integrity of Mitotic Spindles through Maintenance of Kinetochore Factors Mis6/CENP-I and CENP-A
Figure 6
Localization of SPB and kinetochore proteins in kis1-1.
(A) A diagram for SPB and kinetochore proteins examined in this study. (B) Localization of GFP-tagged SPB proteins (Cut12, Pcp1, and Sfi1) in WT and kis1-1 cells at 36°C (6 h). (C) Localization of representative kinetochore proteins shown in (A) in WT and kis1-1 cells. A typical mitotic cell for each GFP-tagged factor is exemplified below with Sid4-CFP and Cnp3-tdTomato. (D) Percentages of interphase cells without GFP or tdTomato localization at kinetochores in WT and kis1-1 are shown for each kinetochore factor (n>50). (E) GFP or tdTomato fluorescence intensity at kinetochores in WT (black) or kis1-1 (gray) cells. Cells were grown at 36°C for 6 h. n≥10. ***p<10−4; ****p<10−6 (Student's t-test). Scale bars, 5 µm.
