The Kinetochore Protein Kis1/Eic1/Mis19 Ensures the Integrity of Mitotic Spindles through Maintenance of Kinetochore Factors Mis6/CENP-I and CENP-A
Figure 9
Spindle organization in kis1-1.
(A) Dis1 was not loaded onto unattached kinetochores in kis1-1. Left: Dis1-GFP was visualized together with Cnp3-tdTomato and Sid4-CFP in WT and kis1-1 cells after growth at 36ยฐC for 6โ9 h. Arrowheads indicate a kinetochore without Dis1-GFP. Right: Percentages of mitotic cells having one or more kinetochores without Dis1-GFP. (B) Mad2-GFP localized to unattached kinetochores in kis1-1. Left: Mad2-GFP in WT and kis1-1 cells was observed with Sid4-CFP and Cnp3-tdTomato after growth at 36ยฐC for 6โ9 h. Arrowheads indicate kinetochores with Mad2-GFP. Right: Percentages of metaphase cells bearing Mad2-GFP dots at kinetochores. n: The number of cells analyzed. Scale bar, 5 ยตm. (C) Ten-fold serial dilutions of the indicated strains spotted on YE5S at 25 or 32ยฐC.
