Negligible Immunogenicity of Induced Pluripotent Stem Cells Derived from Human Skin Fibroblasts
Figure 3
(A) Expression of MHC proteins and costimulatory molecules by hiPSCs treated with 100 ng/ml of IFN-γ for 48h. Gray: isotype controls; Black: positive staining of antigens expressed on hiPSCs without IFN-γ treatment; White: positive staining of antigens expressed on hiPSCs treated with IFN-γ. (B) Concentration dependence of MHC-I induction by IFN-γ in hiPSCs. (C) Time response of MHC-I expression in hiPSCs treated with IFN-γ. (D) MHC-I expression gradually decreased after IFN-γ was withdrawn from the culture medium. Three independent experiments were performed for each analysis.
