Monitoring Brain Tumor Vascular Heamodynamic following Anti-Angiogenic Therapy with Advanced Magnetic Resonance Imaging in Mice
Figure 5
Effects of B20 therapy on glioblastoma tumors.
Tumor volume (mm3) for each individual tumor (A) and the average volume of the entire group (B), as a function of days post cell inoculation for control (black, n = 15) and B20 treated (blue, n = 6) mice. B20 showed only moderate effects on tumor growth kinetics. Comparison of the average diffusion (C) and HRI (D) values of tumors (blue; larger than 15 mm3) and the CLS (red) between control and B20 treated mice. Additionally, the HRI tumor values were also separated between borders (green) and the central area (purple). (E) Quantification of vessel count/HPF analyzed from 10 randomly selected HPF/tumor is based on the CD31 immunostaining. Tumor vascularity is significantly higher in the border (green) compared to the center (purple) in both groups. (F) Representative histological sections of control (left) and B20 treated (right) immunostained with Ki67 for proliferation (top) and TUNEL for apoptosis (bottom); Original magnification X40. *denotes statistical significance at P<0.05; **denotes statistical significance at P<0.01; ***denotes statistical significance at P<0.005.
