Characterization of Dedifferentiating Human Mature Adipocytes from the Visceral and Subcutaneous Fat Compartments: Fibroblast-Activation Protein Alpha and Dipeptidyl Peptidase 4 as Major Components of Matrix Remodeling
Fig 4
Relative gene expression of matrix remodeling genes in dedifferentiating adipocytes.
A) FAP, DPP4, MMP1 and TGFβ1 expression levels were measured in mature adipocytes (D0) and in dedifferentiating adipocytes at days 4 and 7 (D4-D7) of ceiling culture. B) Data are expressed with depot-specific expression at days 0, 4 and 7 for omental (OM) and subcutaneous (SC) cells. C) Comparison of gene expression between adipose tissues, preadipocytes and DFAT cells from the OM and SC fat depots. Data are expressed as mean value of ΔΔCt relative to G6PD expression ± SEM (n = 4). Matched pair t-test: *p<0.05, ** p<0.01, § p<0.10.