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Non-Anticoagulant Fractions of Enoxaparin Suppress Inflammatory Cytokine Release from Peripheral Blood Mononuclear Cells of Allergic Asthmatic Individuals

Fig 4

Effect of fraction 4 on cellular viability.

(A) Viability of PBMCs is presented as mean % LDH release ± SD from allergic asthmatic subjects (n = 3). Cells were incubated for 72 hours in the presence of PHA, fraction 4 or PHA + fraction 4.*p<0.05 and **p<0.01 versus unstimulated control. (B) Viability of PBMCs from allergic asthmatic subjects (n = 3) was determined by trypan blue dye exclusion test and is presented as % of viable cells remaining after 72 hours of incubation with PHA, PHA + fraction 4 or fraction 4 alone. Data is presented as mean ± SD. (C) Proliferation of PBMCs from allergic asthmatic subjects (n = 3) was determined after 72 hours in the presence of PHA, fraction 4 alone or PHA + fraction 4. Cells were counted after incubation for 72 hours and expressed as million cells/mL. Data is presented as mean ± SD.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0128803.g004