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Fluorescent Protein Based FRET Pairs with Improved Dynamic Range for Fluorescence Lifetime Measurements

Table 3

Fluorescence lifetime and FRET efficiency of the FRET pairs inside bacterial cells (From an average of approximately 35 individual cells, for each fluorescent protein/pair).

E—FRET efficiency (calculated according to Eq 3). χ2—calculated standard weighted least squares to assess the goodness of the fit.

Table 3

doi: https://doi.org/10.1371/journal.pone.0134436.t003