Expression and Functional Analysis of WRKY Transcription Factors in Chinese Wild Hazel, Corylus heterophylla Fisch
Fig 5
Expression patterns of WRKY genes after exposure to various abiotic stresses and in different organs/tissues.
The ChActin gene was used as an internal control for qRT-PCR. The y-axis represents relative expression, calculated using the 2−ΔΔCt formula. (A-C) Expression profiles of WRKY genes under cold (4°C), drought (25% PEG6000) and salinity (400mM NaCl) growth conditions, respectively. Leaf samples were collected at 2, 4, 8 and 24h, with the untreated seedlings grown and analyzed in parallel. The expression level values of the genes in untreated seedlings were set to 1.0. (D) Expression patterns of WRKY genes in different organs/tissues, including male anthotaxies, floral buds and stems, which were collected at the same stage. The experiments were repeated with at least three biological and three technical replicates, yielding consistent results.
