The Effector Domain of MARCKS Is a Nuclear Localization Signal that Regulates Cellular PIP2 Levels and Nuclear PIP2 Localization
Fig 1
MARCKS localizes to the nucleus in D54 and U251 glioma cells.
A) D54 and U251 cells were fixed and stained for MARCKS and DAPI with the merged image shown on the right side as indicated. B) D54 and U251 cell lysates were separated into nuclear and cytoplasmic fractions. The proteins were then were separated by 8% SDS-PAGE and probed for MARCKS, the cytoplasmic marker tubulin, and the nuclear marker, lamin.
