The Effector Domain of MARCKS Is a Nuclear Localization Signal that Regulates Cellular PIP2 Levels and Nuclear PIP2 Localization
Fig 5
Changes in RNA expression and protein levels with ED-deleted MARCKS.
A) After overnight doxycycline-induction of ΔED-MARCKS or WT-MARCKS U87 cells, RNA was collected and ran on NanoString nCounter GX Human Cancer Reference Kit. Data was analyzed on nSolver software. Violin plot displays > 1.5 log fold increase in GBM cells overexpressing an ΔED-MARCKS protein as compared to WT MARCKS. B) Changes in total protein levels as well as phosphorylated protein levels between the ΔED and WT- MARCKS expressing U87 GBM cell lines were determined by Kinex KAM-850 microarray. Data is displayed as z-ratio of ΔED results compared to WT-MARCKS results. Increase in z-ratio is indicated in green dots while decrease is indicated in red dots. Total protein targets are listed in standard font while phosphorylated proteins are listed in italicized font with phosphorylated amino acids designated.
