Acute Heat Stress and Reduced Nutrient Intake Alter Intestinal Proteomic Profile and Gene Expression in Pigs
Fig 1
A representative 2D-DIGE gel from the ileum showing identified proteins.
A total of 45 μg of CyDye labeled protein (15 μg of each CyDye 2, 3, and 5) was loaded onto a 11 cm pH 3–10 IPG strip for the first dimension and the second dimension was run on a 12.5% SDS-PAGE gel. Proteins labeled with CyDye5 are shown.