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Flexible Sheet-Type Sensor for Noninvasive Measurement of Cellular Oxygen Metabolism on a Culture Dish

Fig 1

Schematic diagram of flexible sensor sheet.

During the measurement, the device is attached to the bottom of the culture dish to form a temporarily closed microspace around the target cells, hence enabling the short-time evaluation of oxygen consumption rate. The device comprises a transparent EVOH/PDMS sheet and an array of microchamber structures (φ 90 μm, 50 μm depth) that contain a 1-μm-thick sensing layer at their bottom.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0143774.g001