Flexible Sheet-Type Sensor for Noninvasive Measurement of Cellular Oxygen Metabolism on a Culture Dish
Fig 2
(a) Self-aligned hot embossing method used to fabricate the device. First, a 50-μm-thick EVOH film was laminated onto a PDMS sheet, which was followed by the spin-coating of PtOEP/polystyrene. Subsequently, a silicon micromold was embossed onto the layered polymer under optimized conditions, typically a temperature of 130°C and a force of 10 kN, to form microhole structures containing a self-aligned phosphor sensor layer at their bottom. Finally, the residual phosphor layer outside the hole features was peeled off to obtain the sensor sheet. (b) Experimental setup for in situ measurement of cell oxygen consumption. Both phosphorescence lifetime measurement and cultivated cell imaging were automatically performed. The data acquisition speed of the automated sequential measurement system was 100 points/min.
