Flexible Sheet-Type Sensor for Noninvasive Measurement of Cellular Oxygen Metabolism on a Culture Dish
Fig 3
(a) Photograph of the flexible sensor device. The device comprises a transparent EVOH/PDMS sheet and an array of microchamber structures. (b) Bright-field image and phosphorescence image of the sheet. (c) SEM image of the sheet. Each microchamber has a 90 μm diameter and a 50 μm depth, and contains a 1-μm-thick sensing layer at its bottom. (d) Phosphorescence lifetime τ measurement at three different dissolved oxygen concentrations (Cox = 0.00, 0.08, and 0.24 mmol/L). In the presence of oxygen, τdecreased as a result of the quenching of the excited triplet state by the collision of oxygen molecules with phosphor molecules. (e) Oxygen sensor calibration. τ and Cox exhibited a linear relationship in accordance with the Stern-Volmer equation.
