Study of a High-Yield Cellulase System Created by Heavy-Ion Irradiation-Induced Mutagenesis of Aspergillus niger and Mixed Fermentation with Trichoderma reesei
Fig 5
FPA (A), EG (B), CBH (C) and BGL (D) enzyme activities were determined directly from fermented broth obtained at various time points from mixed cultures of T. reesei My and A. niger H11-1 inoculated at different ratios (5:1, 5:3 and 1:1) and grown in a volume of 50 mL in 250-mL conical flasks with shaking at 30°C for 96 h at 200 rpm.