Study of a High-Yield Cellulase System Created by Heavy-Ion Irradiation-Induced Mutagenesis of Aspergillus niger and Mixed Fermentation with Trichoderma reesei
Fig 7
Final FPA, EG, CBH and BGL enzyme activities were determined directly from fermented broth obtained at various time points from monocultures (T. reesei My or A. niger H11-1) and mixed cultures (T. reesei My and A. niger H11-1) grown in a volume of 50 mL in 250-mL conical flasks with shaking at 30°C for 96 h at 200 rpm.
The inoculum volume of T. reesei or A. niger was 2.5 mL for monocultures; the inoculum ratio of T. reesei to A. niger was 5:1 for the mixed cultures, with simultaneous inoculation.
