The Cell Death Inhibitor ARC Is Induced in a Tissue-Specific Manner by Deletion of the Tumor Suppressor Gene Men1, but Not Required for Tumor Development and Growth
Fig 3
ARC protein is more abundant in β-cells of Pdx1-Cre; Men1 f/f mice.
A) Immunostaining of pancreatic tissue for ARC from 12 m old mice. Nuclei counterstained with DAPI. Scale bar denotes 50 μM. B) Immunoblot (top) of islets isolated from Men1 f/f or Pdx1-Cre; Men1 f/f mice. Each lane represents islets isolated from an individual mouse. Quantification of ARC normalized to loading control (bottom). C) Representative FACS analysis of dissociated mouse pancreatic cells stained for ARC and insulin. D) Quantification of ARC-positive non-β-cells (as indicated by absence of insulin staining). E) Quantification of ARC-positive β-cells (insulin positive). N = 3 mice per genotype. **** P < 0.0001 versus each group.
