The Succinate Receptor GPR91 Is Involved in Pressure Overload-Induced Ventricular Hypertrophy
Fig 6
ANP gene expression and PI3K/Akt signaling in cardiac muscle cells in vitro.
A: Immunofluorescent images of cardiomyocytes with fluorescein isothiocyanate (FITC)-conjugated siRNA GPR91. B, C: GPR91 gene and protein expression in the sham and siRNA-GPR91 groups (n = 3). D: ANP expression in the sham, succinate, succinate+wortmannin, and succinate+siRNA-GPR91 groups (n = 3). E: Bar graph showng p-Akt/Akt expression as determined by western blot analysis of each group (n = 3). F: p-Akt/Akt expression as determined by western blot analysis of each group (n = 3); *P<0.05 compared to the sham group; #P<0.05 compared to the succinate group.
