The Fifth Domain of Beta 2 Glycoprotein I Protects from Natural IgM Mediated Cardiac Ischaemia Reperfusion Injury
Fig 1
Infarction size in C57BL/6 WT mice.
(A) Representative images of myocardial infarction in a mouse treated with control after 30 min ischemia and 24 h reperfusion. Section of myocardium at the mid papillary level stained with Evans Blue and 1% triphenyl-2,3,4- tetrazolium-chloride for 20 min at 37°C. The area stained blue with Evans Blue represents the non AAR (white arrow). The remaining area consists of myocardial infarction staining pale (black arrow) and the surrounding AAR that did not sustain myocardial necrosis. (B) Representative image of myocardial infarction in a mouse treated with rhDomain V. The pale staining area of infarction (black arrow) is significantly smaller than control. Consequently the extent of salvaged myocardium (orange arrow) is significantly larger. Sections photographed using Canon EOS 600D camera with Canon EF 100 mm lens and Macro ring lite MR-14Ex light source. (C) Points representing myocardial infarction size defined by 1% TTC staining. Mice treated with sham LAD occlusion demonstrated no myocardial infarction (n = 3). Control groups treated with normal saline 100μL, no treatment or human β2GPI all had similar infarction size. Mice treated with rhDomain V had a significant reduction in infarction size (n = 8 per group). AAR; Area at Risk. Individual points represent infarct size as a % of AAR of a single mouse. The horizontal line for each study group represents the mean.
