Real-Time Label-Free Embolus Detection Using In Vivo Photoacoustic Flow Cytometry
Fig 4
Embolus dynamics in control mice before, during and after compression and surgery.
(A) Profile plot of average detection rate (emboli per hour) from before, 30 minutes during (red region) and after compression with a clamp (400 g) (7 procedures). Detection-rate values and error bars represent the mean and SEM of detection rates during successive 10-minute time intervals, and are positioned at the intervals’ midpoints. (B) Effects of compression effect on embolus detection rates. Values (error bars) represent the estimates (90% confidence intervals) of detection rates (emboli per hour) before compression vs. during and after compression and during vs. after compression as determined from mixed-models Poisson-regression analysis (7 procedures). (C) Profile plot of average detection rates (emboli per hour) for 30 minutes before and 190 minutes after surgery. Values and error bars represent the mean and SEM of detection rates during successive 10 minute time intervals (N7 procedures). Red arrow indicates initiation of the surgery (duration ~3 min). (D) Embolus detection rates in counts per hour before vs. after surgery. Values (error bars) represent estimated detection rates (SEM) (7 procedures).
