Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative Study
Fig 1
Transcription rate (TR) and mRNA accumulation (RA) of ENA1 upon high pH stimulation.
The transcription rate (open squares) and mRNA amount (closed squares) for the ENA1 gene under alkaline stress was obtained from GRO experiments [27]. In order to convert the radioactive intensities into real units, the mRNA concentrations and mRNA half-lives for the most expressed genes published in (Wang et al., 2002) were compared with radioactive intensities of RA and TR for the same genes at time 0 (steady-state conditions) to obtain a conversion factor. The conversion factor was applied to the complete set of GRO data under alkaline stress. ENA1 data were extracted from this set and represented as the average ± SEM of three experiments.
