Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative Study
Fig 2
Time-course of the subcellular distribution of Crz1 upon high pH stress.
A) Cultures of strain SP039 (CRZ1:GFP-kanMX6 SIK1:mRFP-kanMX6) were shifted from pH 5.5 to pH 8.0 and the localization of Crz1 monitored by fluorescence confocal microscopy as described in Materials and Methods. Open circles denote the percentage of cells with nuclear Crz1 localization; closed circles, cytosolic localization. The discontinuous line show cells in which Crz1 entered the nucleus, then left the nucleus and later returned to it (they are, therefore, a subset of the total nuclear Crz1 values). Sik1 signal was used as marker of constitutive nuclear (nucleolar) localization. Data is presented as percentages calculated for 90 to 160 cells examined for each time-point. B) Example of time-lapse images of SP039 cells before and after induction of alkaline stress over a 20 min time-course (as in panel A).
