Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative Study
Fig 5
Time-course profile of Crz1 recruitment at the ENA1 promoter following alkaline stress.
A) Cumulative counts of reads using a 50-nt window spanning the ENA1 promoter region are represented as fold-change over reads counted at time 0. The positions of CDRE deduced from computational analysis using the PWMTools website (http://ccg.vital-it.ch/pwmtools/pwmscan.php) of the Swiss Bioinformatics Institute are indicated as colored boxes on the black line. Red boxes denote the CDRE previously characterized in [23]. B) Evaluation of relative Crz1 binding to CDREs at positions -813/-821 and -719/-727 (red boxes in panel A). Chromatin immunoprecipitation using HA-tagged Crz1 was carried out and the immunoprecipitate used to amplify the -680/792; -771/-917; and -986/-1120 promoter regions (denoted as thick short lines 1, 2 and 3, respectively, in panel A). Data is presented as the mean ± SEM from 3–4 individual experiments assayed in duplicate and expressed as relative to the maximum value.
